Coding

Part:BBa_K5109012

Designed by: Lisa Faccincani   Group: iGEM24_Uni-Padua-IT   (2024-09-27)


Multicopper Oxidase from Synechocystis

This part consists of the sequence encoding Synechocystis PCC 6803's laccase, which is an enzyme selected to be part of a surface display system: BBa_K5109022.

We selected this enzyme because it is endogenously expressed in a microorganism known for its high survival capabilities in PFAS-contaminated environments. Additionally, a BLAST search demonstrated significant similarity to a laccase expressed in Pleurotus ostreatus, which has been shown in previous studies to effectively degrade PFOA and may be applicable for the degradation of other PFAS compounds. A further BLAST analysis revealed its similarity to E. coli's laccase, which we have also selected for our project (BBa_K5109011).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 200
    Illegal AgeI site found at 1077
    Illegal AgeI site found at 1342
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None